Figure 4

Stability of transferrin from a preformed corona on the polymer-coated, negatively charged nanoparticle. FPLC-chromatograms: I, upon transferrin binding the SPIOs with a hydrodynamic diameter of 25 nm increased the volume to about a diameter of 35 nm; II, a second incubation with 125I-albumin resulted in additional binding of 125I-albumin, III, labelling of transferrin on the SPIOs by incubation with 59Fe-NTA (filled area). A, initial transferrin adsorbed; B, initial transferrin covalently bound. The peak at 33 min represents proteins with the size of transferrin (DLS: 9 nm).

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