Figure 6

Bioluminescence imaging of QD-BRET (QD) microinjected cultured ovarian follicles. Follicles were microinjected with QD1− or QD1+ during in vitro culture. Imaging was performed with follicle controls, microinjected with only PBS (Line A, CTL), QD1− (Line A, White Box), or QD1+ (Line A, Red Box), follicles microinjected with both coelenterazine and QD1− (Line B, White Box), or QD1+ (Line B, Red Box). The inset shows follicles microinjected with coelenterazine only (Line C) or with QD1− and QD1+ , showing respective bioluminescence emissions in Lines D and E.

© De Gruyter