Figure 5

Cyclin E accumulates in the nucleus of live Xenopus laevis embryos at the MBT (6 hpf). One cell of the 2-cell embryo was microinjected with in vitro transcribed Myc6-GFP-Cyclin E RNA and the translated protein visualized in live embryos using confocal microscopy in real time. (a, b) Fluorescence channel, Z stack images #5 and #8 from the top, respectively. Nuclei are marked with white arrowheads. (c) Light field image. A 3D image is shown. Scale bars are 100 μM. Embryos were viewed with a 10X objective on a Zeiss LSM 510 confocal microscope equipped with a META detector, and analyzed using LSM510 Image Acquisition software. At least 20 embryos were injected in at least 3 separate experiments.

© De Gruyter