Figure 5

SNOM image of the head and midpiece of a human spermatozoon. (A and B) SNOM 3D representation and topography, (C) longitudinal profile along the head as shown by the white line in (A); (D) SNOM optical reflection and (E) transmission. In (D) black arrows indicate the presence of a faint bright layer around the head, this layer is well-defined and its ends are indicated by the red arrows, while white arrow indicates the nuclear region. In (F) horizontal cross section profile along the white line that allows evaluating the width of the bright layer around the head.

© De Gruyter