Fig. 7

Colocalization of SeNPs@Am and lysosomes in HepG2 cells. a HepG2 cells were treated with lysosomal marker lyso tracker red (red fluorescence) and coumarin-6-loaded SeNPs@Am (green fluorescence) at 37 oC for different time and visualized under a fluorescence microscope (magnification, ×400). b In vitro release profile of Am from SeNPs@Am in RPMI-1640 medium with 10 % fetal bovine serum. Am concentrations were determined by HPLC analysis. c Cellular amount of Se in HepG2 cells after 4 h of incubation with SeNPs@Am. The cells were incubated for 4 h either at 37 °C (control) or at 4 °C. Prior to the incubation with SeNPs@Am, the cells were pretreated with specific endocytosis inhibitors for 1 h. **p < 0.01, ***p < 0.001 vs. the control

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