A schematic representation of quantum dot based single cell imaging cytometry for the determination of breast cancer subtypes. Biopsied tissues or primary cells from breast tumors were treated with four different quantum dot-biomarker (EGFR1, HER2, ER, and PR) conjugates and excited by UV light. Hyperspectral fluorescence cellular images were detected at 525 nm, 565 nm, 605 nm, and 655 nm with the help of acousto-optic tunable filter (AOTF). The optical spectrum represents increasing wavelength as a function of color i.e. blue to red. BF: bright field .