Figure 9:

(A) (a) A calibration curve for wild-type VSV (8G5) sample tested with IRIS platform. Corresponding height changes upon specific virion particle binding is shown. Greater binding indicates the greater affinity to immobilized antibody on the sensor. (b) Specific binding and low non-specific binding are shown in the results of pre- and post-incubations with the virions. Adapted from [87]. (B) (a) A SP-IRIS microarray configuration with immobilized antibody spots in green, red, and blue for anti-VSV, anti-EBOV, and anti-MARV probes, respectively. (b) Data acquisition and analysis for virus identification using SP-IRIS. Captured VSV particles can be seen on anti-VSV antibody spotted sensor surface (right) when compared to pre-incubation image (left). (c) The model used for determining the size of each particle within the spot (left) and a size distribution of particles identified on the spot of the image (right). The selected region on the plot represents expected sizes of VSV. Adapted from [88] (Reprinted with permission from [88]. Copyright 2014 American Chemical Society). (C) (a) Schematic representation of the SP-IRIS detection principle (b) SP-IRIS signal for polystyrene nanoparticles with a diameter from 50 to 200 nm used to be reference for exosomes. (c) An image of the SP-IRIS chip. (d) IRIS image of immobilized antibody surface probes. (e) SP-IRIS image of captured particles on the spot, which are detected via NVDX analysis. Adapted from [38]. (D) SP-IRIS measurement of spherical gold nanoparticles (r=30 nm) at (a) h=0 nm (GNP1) and h=40 nm (GNP2), and (b) their interferometric responses (GNP1 shown in red, and GNP2 shown in blue). Adapted from [89].

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