Figure 5:

(A) The single particle interference reflectance imaging sensor (SP-IRIS) instrument layout and (B) schematic of substrate and surface coatings. Wide-field epi-illumination is reflected by the thin film substrate, and individual BNPs are detected as diffraction-limited perturbations to the reflected field. Beyond label-free detection of individual BNPs, functionalized metallic nanoparticles may be used as single-molecule labels. Adapted from [24].

© De Gruyter