Chitosan nanoparticles based nanovaccines for cancer immunotherapy
Pu-Guang Chen
/ Zhi-Hua Huang
/ Zhan-Yi Sun
/ Yue Gao
/ Yan-Fang Liu
/ Lei Shi
/ Yong-Xiang Chen
/ Yu-Fen Zhao
/
Yan-Mei Li1
Department of Chemistry, Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology (Ministry of Education), Tsinghua University, Beijing, P. R. China
2
Department of Chemistry, Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology (Ministry of Education), Tsinghua University, Beijing 100084, P.R. China. Fax: (+86)10-62781695
Cite as: Pu-Guang Chen, Zhi-Hua Huang, Zhan-Yi Sun, Yue Gao, et al. Chitosan nanoparticles based nanovaccines for cancer immunotherapy, nano Online. (2018). DOI: https://doi.org/10.1515/nano.0071.00001
Cite as: Pu-Guang Chen, Zhi-Hua Huang, Zhan-Yi Sun, Yue Gao, et al.. Chitosan nanoparticles based nanovaccines for cancer immunotherapy, Pure and Applied Chemistry. 89, 931 (2017). DOI: https://doi.org/10.1515/pac-2016-0913
Abstract
Cancer immunotherapy based on tumor vaccine is very promising and intriguing for carcinoma treatment. Herein, antitumor nanovaccines consisting of self-assembled chitosan (CS) nanoparticles and two-component mucin1 (MUC1) glycopeptide antigens were reported. Two different kinds of polyanionic electrolyte [sodium tripolyphosphate (TPP) and γ-poly-L-glutamic acid (γ-PGA)] were combined with chitosan polymers to fabricate the diameter of nearly 400–500 nm CS nanoparticles by electrostatic interactions. The nanovaccines were constructed by physically mixing MUC1 glycopeptide antigens with CS nanoparticles, which reduced vaccine constructing complexity compared with traditional chemical total synthetic vaccines. Immunological studies revealed that the CS/γ-PGA nanoparticle could dramatically enhance the immunogenicity of peptide epitope and produce significantly high titers of IgG antibody which was even better than Freund’s adjuvant-containing vaccines.
Keywords: cancer immunotherapy; chitosan nanoparticle; ICS-28; IgG antibody; immune response; MUC1 glycopeptide; nanovaccine; peptide antigen
