NADH oxidation in a microreactor catalysed by ADH immobilised on γ-Fe2O3 nanoparticles
1Faculty of Chemical Engineering and Technology, University of Zagreb, Marulićev trg 19, HR-10000 Zagreb, Croatia
2Faculty of Chemistry and Chemical Engineering, University of Maribor, Smetanova ulica 17, SI-2000 Maribor, Slovenia
Cite as: Anita Šalić, Katarina Pindrić, Gordana Hojnik Podrepšek, Maja Leitgeb. NADH oxidation in a microreactor catalysed by ADH immobilised on γ-Fe2O3 nanoparticles, nano Online. (2016). DOI: https://doi.org/10.1515/nano.0015.00005
Cite as: Anita Šalić, Katarina Pindrić, Gordana Hojnik Podrepšek, Maja Leitgeb. NADH oxidation in a microreactor catalysed by ADH immobilised on γ-Fe2O3 nanoparticles, Green Processing and Synthesis. 2, 569 (2013). DOI: https://doi.org/10.1515/gps-2013-0084
Abstract
A new concept of nicotinamide adenine dinucleotide hydrate (NADH) oxidation which combines advantages of the microreactor technology with the advantages of magnetic nanoparticles (MNPs) application is developed. Acetaldehyde was used as a substrate for the NADH regeneration process while the reaction was performed in a batch reactor and in a microreactor using alcohol dehydrogenase (ADH)-loaded MNPs. Three different microreactor systems with MNPs were studied, two with stationary MNPs trapped on the inner surface of microchannel by permanent magnetic field and one where the MNPs actively moved across the channel (movement inside microchannel allowed by an oscillating magnetic field). In a reactor system with an oscillating magnetic field and an actively moving ADH-loaded MNPs 100% NADH conversion was achieved for residence time of just 2 min.
Keywords: alcohol dehydrogenase; coenzyme regeneration; enzyme immobilisation; microreactor; maghemite nanoparticle
