Magnetic spectroscopy of nanoparticle Brownian motion measurement of microenvironment matrix rigidity
1Department of Radiology, Dartmouth Medical School and Dartmouth-Hitchcock Medical Center, Lebanon, New Hampshire, USA
2Thayer School of Engineering, Dartmouth College, Hanover, New Hampshire, USA
3Department of Physics, Dartmouth College, Hanover, New Hampshire, USA
Quellenangabe: John B. Weaver, Kristen M. Rauwerdink, Adam M. Rauwerdink, Irina M. Perreard. Magnetic spectroscopy of nanoparticle Brownian motion measurement of microenvironment matrix rigidity, nano Online. (2016). DOI: https://doi.org/10.1515/nano.0007.00015
Quellenangabe: John B. Weaver, Kristen M. Rauwerdink, Adam M. Rauwerdink, Irina M. Perreard. Magnetic spectroscopy of nanoparticle Brownian motion measurement of microenvironment matrix rigidity, Biomedical Engineering / Biomedizinische Technik. 58, 547 (2013). DOI: https://doi.org/10.1515/bmt-2013-0012
Abstract
The rigidity of the extracellular matrix and of the integrin links to the cytoskeleton regulates signaling cascades, controlling critical aspects of cancer progression including metastasis and angiogenesis. We demonstrate that the matrix stiffness can be monitored using magnetic spectroscopy of nanoparticle Brownian motion (MSB). We measured the MSB signal from nanoparticles bound to large dextran polymers. The number of glutaraldehyde induced cross-links was used as a surrogate for material stiffness. There was a highly statistically significant change in the MSB signal with the number of cross-links especially prominent at higher frequencies. The p-values were all highly significant. We conclude that the MSB signal can be used to identify and monitor changes in the stiffness of the local matrix to which the nanoparticles are bound.
Keywords: magnetic nanoparticle spectroscopy; magnetization harmonics; relaxation times; rotational Brownian motion
